Fig. 6: Translation initiation is impaired in axons of Hnrnpr−/− motoneurons.
From: hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis
a Representative images of Pabp-eIF4G PLA signal in cultured Hnrnpr−/− and +/+ motoneurons at DIV 6 using anti-Pabp and anti-eIF4G antibodies. Scale bars, 10 µm and 5 µm (magnified areas). b, SuperPlots of the relative number of Pabp-eIF4G PLA punctae in somata and 50 µm of axons of cultured Hnrnpr−/− and +/+ motoneurons. Statistical analysis was performed using an unpaired two-tailed Student’s t test. Data are mean ± s.d. of three biological replicates. Hnrnpr+/+, n = 44; Hnrnpr−/−, n = 51 motoneurons. c Representative images of eS6-eL24 PLA signal in cultured Hnrnpr−/− and +/+ motoneurons at DIV 6 using anti-eS6 and anti-eL24 antibodies. Scale bars, 10 µm and 5 µm (magnified areas). d SuperPlots of the relative eS6-eL24 PLA signal intensity in somata and number of punctae in 50 µm of axons of cultured Hnrnpr−/− and +/+ motoneurons. Statistical analysis was performed using an unpaired two-tailed Student’s t test. Data are mean ± s.d. of three biological replicates. Hnrnpr+/+, n = 44; Hnrnpr−/−, n = 41 motoneurons. e Representative images of Y10b immunostaining in cultured Hnrnpr−/− and +/+ motoneurons at DIV 6. Scale bars, 10 µm and 5 µm (magnified areas). f SuperPlots of the relative Y10b immunosignal intensity in somata and proximal and distal axonal regions of cultured Hnrnpr−/− and +/+ motoneurons. Statistical analysis was performed using an unpaired two-tailed Student’s t test. Data are mean ± s.d. of three biological replicates. Hnrnpr+/+, n = 97; Hnrnpr−/−, n = 78 motoneurons. Source data are provided as a Source Data file.
