Fig. 1: Spores of the Bacillus cereus group persist in the Drosophila intestine.

a Experimental setup to assess bacterial load after a continuous ingestion of spores. b Bacterial loads of dissected midguts after continuous ingestion of spores from Btk or Bc strains. The dot indicates the mean number of colony-forming units (CFUs) of at least three independent experiments per condition and time point. Each experiment corresponds to the mean of five midguts. CFUs correspond to spore and vegetative cell counts. Error bars correspond to the SEM. Source data are provided as a Source Data file. c Experimental setup to assess bacterial load after an acute ingestion of spores. Flies are in contact with the contaminated medium for 30 min and then transferred to fresh vial devoid of spores. d Bacterial loads of dissected midguts after acute ingestion of spores from Btk or Bc strains. The dot indicates the mean number of CFUs (spores + vegetative cells) of at least three independent experiments per condition and time point. Each experiment corresponds to the mean of five midguts. Error bars correspond to the SEM. * represent a statistically significant difference (p < 0.05) between Bc Bactisubtil and the other strains 10 days post-feeding using the two-sided Non-parametric Mann–Whitney’s test against each individual condition at 240 h. Source data are provided as a Source Data file. e Bacterial loads in split Drosophila midguts after acute intoxication with Btk (SA-11) or Bc ATCC 14579 spores. Dots correspond to independent experiments and are the mean of five pooled midgut domains. Error bars correspond to the SEM. The one-side Mann–Whitney tests were applied. Asterisks represent a statistically significant difference between bacterial loads in the anterior and the posterior midguts: **p < 0.01, *p < 0.05. Source data are provided as a Source Data file.