Fig. 2: Single-cell RNA-sequencing overview and cell composition analysis of ICI-resistant melanoma tumors treated with cryoablation.

A Overview of the scRNA-seq cohort. Visual production credits “iStock.com/MaskaRad”. B Summary of available samples by patient and response status, organized by anatomical site and labeled by timing obtained (i.e pre-tx: pre-ablation, pre+post tx: pre-ablation and post-ablation). C Uniform manifold approximation and projection (UMAP) plot of all single cells passing quality control filtration, colored by cell lineage. D UMAP expression density plots of the following lineage-defining marker genes (left to right, top to bottom): PMEL, VWF, CD3E, LYZ, CD79A, COL1A1. E Box plots displaying (top) the percentage of T, B/plasma, and myeloid cells out of all CD45+ cells, and (bottom) the percentage of melanocyte, endothelial, and stromal cells out of CD45- cells for pre-treatment samples only. Box plots are grouped by ultimate response to cryoablation, comparing patients with progressive disease (n = 10) to those without (n = 3). Box plots indicate interquartile range, with horizontal line indicating median, and whiskers extending to maxima and minima excluding outliers (>1.5 * IQR). Indicated p values are for the Kruskal–Wallis test, uncorrected. PD progressive disease, SD stable disease, PR partial response. Source data for Fig. 2 are provided as a Source Data file.