Fig. 1: Transcriptome analysis of differentially expressed genes (DEGs) induced by RBSDV between the two NILs. | Nature Communications

Fig. 1: Transcriptome analysis of differentially expressed genes (DEGs) induced by RBSDV between the two NILs.

From: ZmGDIα-hel counters the RBSDV-induced reduction of active gibberellins to alleviate maize rough dwarf virus disease

Fig. 1

a, b Relative expression of RBSDV S10 and S7-1 (n = 3) between the two NILs 16 days post RBSDV inoculation (dpi). A biological replicate in the NIL-R serves as a control sample for relative expression. c A volcano plot depicts the DEGs when comparing NIL-S to NIL-R at 16 dpi. Genes with significant down-regulation are represented by green dots, those with significant up-regulation by red dots, and those without significant change by blue dots. d A heatmap displays the 45 DEGs induced by RBSDV when comparing NIL-S to NIL-R. Red indicates up-regulation, while green denotes down-regulation. The red asterisk denotes ZmGA2ox7.3. e Enrichment of the ZmGA2ox7.3 transcript in NIL-S compared to NIL-R. A structure diagram of ZmGA2ox7.3 is provided at the bottom. f The relative expression of ZmGA2ox7.3 was verified by RT-qPCR (n = 3). A biological replicate in the non-infected NIL-S at 27 dpi serve as a control sample for relative expression. In (d, e), “rep1” and “rep2” indicate two biological replicates. In (a, b, f), data are presented as means ± s.e.m. Significance was determined by a two-tailed Student’s t test.

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