Fig. 6: The formation of oligomer ZmGA2ox7.3 depends on bioactive GA4. | Nature Communications

Fig. 6: The formation of oligomer ZmGA2ox7.3 depends on bioactive GA4.

From: ZmGDIα-hel counters the RBSDV-induced reduction of active gibberellins to alleviate maize rough dwarf virus disease

Fig. 6

a Chromatographic separation of a mixture of five standard proteins. b Plots of calibration curves for standard proteins. “R” indicates the correlation efficiency. c The quantities of purified GST-ZmGA2ox7.3 and GST-ZmGA2ox7.33A. d The gel filtration profiles of ZmGA2ox7.3 and ZmGA2ox7.33A with or without 0.5 mM GA4. The peaks correspond to the tetramer (T), dimer (D), and monomer (M) forms. e The molecular weights of the “T”, “D”, and “M” peaks of ZmGA2ox7.3 in the presence of 0.5 mM GA4. f The eluted “T” peak fractions from ZmGA2ox7.3 and ZmGA2ox7.33A with or without 0.5 mM GA4. g The plant morphology of Col-0, OE-ZmGA2ox7.33A, and OE-ZmGA2ox7.3 in Arabidopsis thaliana. The exogenous ZmGA2ox7.3-GFP3A and ZmGA2ox7.3-GFP proteins were detected using immunoblotting with anti-GFP antibodies. Scar bar, 5 cm. Significance level was determined by a two-sided simple linear regression statistical test (b). The gel filtration chromatography assays were repeated three times.

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