Fig. 4: METTL3/METTL14 maintain nucleoli integrity depending on their core motifs.
From: METTL3/METTL14 maintain human nucleoli integrity by mediating SUV39H1/H2 degradation
a Upper panel, Morphology of METTL3 deficient cells (METTL3-OE/KO/DOX-) rescued by WT or mutant METTL3 as indicated. Below panel, Morphology of METTL14 deficient cells (METTL14-OE/KO/DOX-) rescued by WT or mutant METTL14 as indicated. Scale bar, 200 μm. b Cell cycle analysis and qRT-PCR analysis on the expression level of pre-rRNA in the indicated cells in (a). The data represent mean ± SD from three independent experiments (n = 3). Statistical analysis, unpaired two-tailed Student’s t-test. c Immunostaining analysis on NPM1 and the quantification of nucleolus number in the indicated cell lines in (a). Scale bar, 10 μm. The data represent mean ± SD from five independent experiments (n = 5). d Immunostaining analysis on METTL3 or METTL14 and NPM1 in indicated cells in (a). Scale bar, 3 μm. e Upper panel, the important domains in METTL3 protein. Below panel, immunostaining analysis on METTL3 and NPM1 in the indicated cell lines. Scale bar, 3 μm. f Immunostaining analysis on NPM1 and the quantification of nucleolus number in the indicated cell lines in (e). Scale bar, 10 μm. The data represent mean ± SD from five independent experiments (n = 5). g Immunostaining on FBL and NPM1 in the indicated cell lines in (e). Scale bar, 3 μm.
