Fig. 7: Both Por39 and Por41 contribute to PapS localization. | Nature Communications

Fig. 7: Both Por39 and Por41 contribute to PapS localization.

From: An outer membrane porin-lipoprotein complex modulates elongasome movement to establish cell curvature in Rhodospirillum rubrum

Fig. 7

a Morphology of R. rubrum cells producing mutant porin variants with exchanges in the predicted PapS binding site. Shown are por39mut (SP235), por41mut (SP234), and por39mut por41mut (SP242) cells, visualized by phase-contrast microscopy. Bar: 5 µm. b Superplots showing the distribution of cell sinuosities in populations in the indicated R. rubrum strains. The data are presented as described for Fig. 1f. The statistical significance (p value) of differences between strains is indicated (unpaired two-sided Welch’s t-test). c Colocalization of PapS-mNG and the indicated mCh-Por39 variants in the por39mut (LMS23), por41mut (LMS24) and por39/41mut (SP243) backgrounds. Shown are representative cells (n = 3 biological replicates), analyzed by widefield epifluorescence microscopy. Bar: 5 µm. (d) SDS gel showing the presence or absence of porin-PapS complexes in Triton extracts of the indicated R. rubrum strains. Source data are provided as a Source Data file.

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