Fig. 8: PapS promotes the enrichment of elongasome complexes at the outer curve. | Nature Communications

Fig. 8: PapS promotes the enrichment of elongasome complexes at the outer curve.

From: An outer membrane porin-lipoprotein complex modulates elongasome movement to establish cell curvature in Rhodospirillum rubrum

Fig. 8

a, b Muropeptide profiles of R. rubrum wild-type (S1) and ΔpapS (JR52) cells. The major muropeptide species are annotated. “Tetra” stands for N-acetyl-glucosamine-N-acetylmuramitol tetrapeptide. “Anh” indicates Tetra derivatives containing a 1,6-anhydro-N-acetylmuramic acid moiety. c Localization of mNG-RodZ in the wild-type (SP160) and ΔpapS (SP162) backgrounds by widefield epifluorescence microscopy. Bar: 5 µm. Shown are representative cells (n = 3 biological replicates). d, e Demographs showing the distribution of mNG-RodZ fluorescence along the outer and inner curve in populations of wild-type (SP160; n = 511 cells) and ΔpapS (SP162; n = 670 cells) cells. The outer- and inner-curve fluorescence intensity profiles obtained for individual cells were concatenated (as exemplified in (e)) and stacked on top of each other according to their combined length. f Colocalization of PapS-mCh with mNG-RodZ in the wild-type background (SP163). Shown are representative cells (n = 3 biological replicates) cultivated in the absence (untreated) or presence (+) of cefalexin and analyzed by widefield fluorescence microscopy or 3D-SIM, respectively. Bar: 5 µm. The area overlap of the PapS-mCh and mNG-RodZ signals in the 3D-SIM images is 57.9% (see also Supplementary Data 4). g Superplots showing the outer-versus-inner-curve mNG-RodZ signal ratios in the wild-type (SP160) and ΔpapS (SP162) backgrounds. The data are presented as described in Fig. 1f. The statistical significance (p value) of differences between strains is indicated (unpaired two-sided Welch’s t-test). Source data are provided as a Source Data file.

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