Table 1 Kinetic parameters for wild type and mutant PFKL

From: Structural basis for allosteric regulation of human phosphofructokinase-1

 

Km,F6P ± SEM (mM)

Hill coefficient ± SEM

Vmax ± SEM (Units per mg PFK1)

PFKL (n = 7)

2.1 ± 0.3

2.6 ± 0.6

9.6 ± 1.4

PFKL-N702T (n = 6)

1.5 ± 0.1

3.9 ± 0.7

13.8 ± 0.8

PFKL-∆C (n = 5)

0.8 ± 0.1

2.6 ± 0.5

10.6 ± 0.7

  1. The kinetic parameters for PFKL were determined at pH 7.5 with 125 µM ATP. The parameters were generated by fitting data to the allosteric sigmoidal model. The number of experimental replicates is noted in parentheses.
Back to article page