Fig. 2: Genomic characterization of longitudinal samples from sotorasib-resistant KRASG12C LUAD.
From: RAS-ON inhibition overcomes clinical resistance to KRAS G12C-OFF covalent blockade
A Schematic overview of the longitudinal samples. B Heatmap showing the variant allelic frequency (VAF) of somatic mutations present in at least two of the four longitudinal samples. The top annotation heatmap depicts the type of mutation, the cognate damaging effect according to CancerVar (i.e., tier), and the classification of the corresponding gene as cancer genes of lung or other tumors according to the Network of Cancer Genes. C Scatter plots show the VAF of selected mutations across the longitudinal samples. D View of chromosomal amplifications and losses in the longitudinal samples. Oncogenes (red) and tumor suppressor genes (blue) undergoing amplifications and losses in at least one sample are shown. Loss of heterozygosity (LOH) events are depicted in yellow. E Reconstruction of clonal evolution across the longitudinal samples. The evolution of the major clones and minor subclones are shown as colored branches. Tree nodes show samples containing the corresponding clones. F Fish plot visualizes clonal evolution across longitudinal samples. Color codes represent clones detected as in (E). Source data are provided as a Source Data file.
