Fig. 7: LOXHD1 interacts in vitro with MET channel complex and tip link proteins. | Nature Communications

Fig. 7: LOXHD1 interacts in vitro with MET channel complex and tip link proteins.

From: LOXHD1 is indispensable for maintaining TMC1 auditory mechanosensitive channels at the site of force transmission

Fig. 7

a–d Co-immunoprecipitation experiments from HEK293T cells overexpressing LOXHD1-HA with tagged channel complex or tip link proteins. FLAG-TMC1 was co-immunoprecipitated with LOXHD1-HA using anti-HA magnetic beads, but FLAG-TMC2 was not (a); CIB2-V5 (b), FLAG-LHFPL5 (c) and PCDH15 (d) were co-immunoprecipitated with LOXHD1-HA, while TMIE-FLAG was not (b). Each experiment was replicated at least thrice; (a) was replicated five times. Predicted protein sizes based on primary sequence: LOXHD1-HA: 261 kDa; TMC1-FLAG: 88 kDa; TMC2-FLAG: 102 kDa; CIB2-V5: 27 kDa; HA-CIB2: 23 kDa; TMIE-FLAG: 18 kDa; FLAG-LHFPL5: 25 kDa; PCDH15: 198 kDa. m: monomeric protein molecular size; mm: multimeric molecular size; IP: Immunoprecipitation. For corresponding full blots, see Supplementary Fig. 7.

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