Fig. 2: Cell-type-specific activation of the 5-HT2A receptor pathway and modulations of spontaneous activity. | Nature Communications

Fig. 2: Cell-type-specific activation of the 5-HT2A receptor pathway and modulations of spontaneous activity.

From: Gain control of sensory input across polysynaptic circuitries in mouse visual cortex by a single G protein-coupled receptor type (5-HT2A)

Fig. 2

a Photostimulation of the 5-HT2A receptor pathway in pyramidal neurons. Left: Scheme of paradigm. Middle: spontaneous activity of excitatory neurons upon photostimulation (Sph, dark orange), and under control condition (S, gray (throughout figure)). Blue bar shows the photostimulation time. Right: same conditions for the recorded pool of inhibitory neurons. Data represent mean ± SEM (shadings) of n = 55 excitatory units and n = 44 inhibitory units in 11 NEX-Cre mice. b Quantification (mean, error bars show + SEM) of the normalized firing rates in (a) in the time interval of 10–13 s (marked as ‘POST’ in a), (color scheme as in a). c Violin plots of opto-index (OI, see formula) values of all excitatory (dark orange circles) and inhibitory (light blue circles) neurons presented in (a, b), with kernel density estimation for the two populations; the left plot shows values for controls using OI-congruent PRE and POST times. d Activity of excitatory neurons (dark orange) with positive OI (solid line, SphOI+, n = 39) and negative OI (stippled line, SphOI−, n = 16) and activity of inhibitory neurons (light blue; SphOI+, n = 21, SphOI−, n = 22). Data represent mean ± SEM (shadings). e Data in (d) quantified as in (b) (color scheme as in d). f Photostimulation of the 5-HT2A receptor pathway in PV interneurons. Middle: pool of inhibitory neurons during control condition (S, gray) and following photostimulation (Sph, dark blue). Right: pool of excitatory neurons recorded under same conditions, control (gray) and with photostimulation (light orange). Data represents mean ± SEM (shadings) of n = 73 inhibitory units (37 with OI+, 36 with OI−) and n = 56 excitatory units (6 with OI+, 50 with OI−) recorded in 14 PV-Cre mice. g Quantification of normalized firing rates in (f) (averaged over same time interval as in a). (h, i, j) Same analysis and conventions as in (c–e) for data shown in (f). ***p < 0.001, **p < 0.01, and *p < 0.05, two-sided paired sample t-test in (b and g), one-sided in (e and j) or two-sample Kolmogorov–Smirnov test in (h). Exact p-values of all comparisons are reported in the Source Data file.

Back to article page