Fig. 4: KaiC3 and KaiC1 protein abundance and phosphorylation profiles in Synechocystis cells grown under continuous illumination conditions after synchronization.
From: Two KaiABC systems control circadian oscillations in one cyanobacterium
a Western blots showing KaiC3 and KaiC1 phosphorylation over three days. The cells were cultured in liquid medium, entrained with two LD cycles, and then returned to LL. Cells were harvested every 4 h at LL. 8 μg of total protein were loaded in each lane and subjected to western blot analysis. We designated the upper bands as hyperphosphorylated (PP-KaiC) and the lower bands as hypophosphorylated (P- and NP-KaiC), analogous to our in vitro data and the Synechococcus KaiC, and based on Fig. S9b. The experiment was performed once over three LL cycles and confirmed once over one LL cycle. b Densitometric estimation of rhythms in the KaiC3 and KaiC1 fractions of PP-KaiC (left) and KaiC protein abundance (right) from the blots shown in (a). Source data are provided as a Source Data file.
