Fig. 2: Dopamine recognition of VMAT2.
From: Neurotransmitter recognition by human vesicular monoamine transporter 2
a, b Electrostatic surface representation of VMAT2dop highlighting the substrate-binding pocket. Side view (a) and top view (b). c Top view of the structural superposition of VMAT2dop (orange) and the outward-facing conformation of GLUT3 (cyan, 4ZWC). Dopamine of VMAT2 and maltose of GLUT3 are indicated by green and gray sticks. d Detailed interaction between dopamine and VMAT2. Dopamine and contact residues are shown as green and orange sticks. e Schematic representation of dopamine-binding interaction. Hydrogen bonds and salt bridge are shown as dashed lines. f Uptake of dopamine by proteoliposomes containing purified VMAT2 variants. Error bars represent mean ± SEM; points represent biologically independent measurements (n = 6 for WT and D399A; n = 5 for others). P values for differences between wild-type and variants were obtained from a one-way analysis of variance (ANOVA) followed by the Dunnett’s multiple comparisons test. *P < 0.01; from left to right, P < 0.0001, P < 0.0001, P < 0.0001, P < 0.0001, and P = 0.0003.
