Fig. 1: Vitamin A restores neuronal differentiation by reducing lipid peroxidation.
a Scheme of neuronal differentiation using three conditions (i) standard media with antioxidant-containing B27 ( + AO), (ii) media with antioxidant-deficient B27 containing vitamin A (-AO + vAlo), or (iii) media with antioxidant-deficient B27 containing vitamin A, which was further supplemented with retinoic acid (RA) (-AO + vAhi). b mRNA levels of RARB at day 20 of cortical neuronal differentiation using quantitative RT-PCR. Data are mean ± SD of n = 3 biologically independent replicates, one-way ANOVA with Tukey’s test. c C11-BODIPY staining of day 20 immature cortical neurons supplemented with vitamin A using flow cytometry (n = 4). (Left) flow cytometry histograms; (right) median intensity. Data are mean ± SD of n = 4 biologically independent replicates; one-way ANOVA with Tukey’s test. d (Left) MAP2 immunofluorescence staining of cortical neurons at day 40. Scale bars, 100 µm. (Right) high-content image analyses. n = 3 biologically independent replicates with every 2 technical replicates; one-way-ANOVA with Tukey’s test. Source data are provided as a Source Data file.
