Fig. 4: Vitamin A reduces iron-overload-mediated developmental growth defect in C. elegans and suppresses ferroptosis in HT-1080 cells. | Nature Communications

Fig. 4: Vitamin A reduces iron-overload-mediated developmental growth defect in C. elegans and suppresses ferroptosis in HT-1080 cells.

From: Suppression of ferroptosis by vitamin A or radical-trapping antioxidants is essential for neuronal development

Fig. 4: Vitamin A reduces iron-overload-mediated developmental growth defect in C. elegans and suppresses ferroptosis in HT-1080 cells.

a Box plot showing the relative worm length at 72 h of development in control, FAC-treated or FAC-treated supplemented with Trolox or vitamin A animals. Data are from 4 biologically independent replicates (15–30 worms per biological replicate). The median is a thick line. Box limits are 25th and 75th percentiles, and whiskers denote 1.5 times the interquartile ranges. p values were calculated with a Wilcoxon rank sum test with continuity correction (two-sided). b CellTiter-Glo assay of HT-1080 cells co-treated with RSL3 and ATRA (n = 3 biologically independent replicates). Data shown are mean ± SD. c C11-BODIPY cell-free assay of the radical initiator 2,2’-Azobis(2-amidinopropane) dihydrochloride (AAPH) co-treated with Fer-1 or ATRA. Data are mean ± SD of n = 3 biologically independent replicates; one-way-ANOVA with Tukey’s test. d (Left), HT-1080 spheroids co-treated with RSL3 and Fer-1, ATRA, or in combination with inhibitors of Retinoic Acid Receptor (RARi) and Retinoid X receptor (RXRi). (n = 13; remaining spheroids in Supplementary Fig. 5) (Right), quantification of spheroid roundness. Data are mean ± SD of n = 13 independent spheroids; Kruskal-Wallis with Dunn’s test. e CellTiter-Glo assay of HT-1080 cells co-treated with RSL3 and ATRA, Retinol or Retinal (n = 3 biologically independent replicates) – Retinal and Retinol at concentrations > 3 µM were toxic. Data shown are mean ± SD. f C11-BODIPY cell-free assay of the radical initiator AAPH co-treated with ATRA, Retinol or Retinal. Data are mean ± SD of n = 6 biologically independent replicates; one-way-ANOVA with Tukey’s test. g CellTiter-Glo assay of HT-1080 cells after co-treatment with RSL3 and Retinol (2 µM) as well as different concentrations of RARi (µM). Data are mean ± SD of n = 3 biologically independent replicates; one-way-ANOVA with Tukey’s test. h CellTiter-Glo assay of HT-1080 cells co-treated with RSL3 and Retinal (0.8 µM) as well as different concentrations of RARi (µM). Data are mean ± SD of n = 3 biologically independent replicates; one-way-ANOVA with Tukey’s test. Source data are provided as a Source Data file.

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