Fig. 2: smFRET captures the conformational dynamics of unfolded OmpX in the presence or absence of SurA variants.
From: Dual client binding sites in the ATP-independent chaperone SurA
a Schematic showing OmpX (black line) with donor and acceptor fluorophores on the N- and C-termini (green and red) with example alternate conformers shaded in grey (top), Eraw histogram (middle) and BVA analysis (lower) for apo-OmpX. The solid black line represents the expected standard deviation for a static (ms timescale) FRET population. The white circles show the mean si for bin widths of 0.05 of Eraw. b OmpX + SurA-WT and (c) OmpX + SurA-core with panels as in (a). The schematic of SurA is coloured according to Fig. 1. Source data are provided as a Source Data file.
