Fig. 1: Single-cell analysis of adipocytic lesions.
From: Cellular origin and clonal evolution of human dedifferentiated liposarcoma
A Graphical view of the study roadmap. Fresh samples were collected from patients undergoing surgery for primary untreated DDLPS (N = 11) and other adipocytic tumors (N = 11) and processed for single-cell suspension. For DDLPS, paired samples in the well-differentiated area (WD, white star) and in the dedifferentiated area (DD, black star) were identified on pre-operative computed tomography, defined macroscopically on the surgical specimen, and later confirmed at the microscopic level by expert pathologists. All samples were analyzed by scRNA-seq using the 10X Genomics platform and integrated into a single adipocytic tumor atlas. A total of 102,753 single cells were recovered, clustering within 42 clusters according to Seurat, and used for subsequent analyses. B Uniform Manifold Approximation and Projection (UMAP) of the 10 main cell types identified according to expression of marker genes. C Dot plots showing the expression of 15 marker genes across the 10 cellular clusters. The size of the dot represents the proportion of cells expressing the marker and the spectrum of color indicates the average expression level of the marker (log1p transformed) scaled over cell clusters. D Relative proportion of each cell cluster according to the histological subtypes (Lipoma, N = 1; WDLPS, N = 5; DDLPS, N = 19; DDLPS-WD, N = 10; DDLPS-DD, N = 9). E: Relative proportion of each cell cluster according to Lipoma (N = 1), WDLPS (N = 5), and DDLPS (N = 11) patients. F: Relative proportion of each cell cluster between paired WD and DD samples from DDLPS patients (N = 8 paired samples). Color scales are similar for panels (B, D, E, and F). Source data are provided as a Source Data file.
