Fig. 4: PKN1 and PKN2 deletion in cardiac fibroblasts suppresses the synthesis of collagen. | Nature Communications

Fig. 4: PKN1 and PKN2 deletion in cardiac fibroblasts suppresses the synthesis of collagen.

From: Protein kinase N promotes cardiac fibrosis in heart failure by fibroblast-to-myofibroblast conversion

Fig. 4

a Efficiency of PKN1 and PKN2 knockdown in cardiac fibroblasts as shown via western blot analysis. b Statistical evaluation of (a) (n = 6, biological replicates per group). ****p < 0.0001. c Quantification of the percentage of Ki67-positive cells in αSMA-positive cells (n = 6, biological replicates per group). ns, not significant. Scratch wound healing assay (d) and quantification (e) after 12 h (n = 5, biological replicates per group). ns, not significant; ****p < 0.0001. f, g Gene expression of collagen isoforms 1 and 3 (determined by quantitative real-time polymerase chain reaction; n = 4, biological replicates per group) after 48 h of TGF-β treatment. ns, not significant; ***p = 0.0003 (f). ns, not significant; ***p = 0.0008 (siControl, TGF-β – vs. siControl, TGF-β +); ***p = 0.0002 (siControl, TGF-β + vs. siPKN1/2, TGF-β +) (g). h, i Gene expression of collagen isoforms 1 and 3 (determined by quantitative real-time polymerase chain reaction; n = 6, biological replicates per group) in the infarct area after 28 days of an MI with the ischemia-reperfusion (IR) model. ns, not significant; **p = 0.0028; ***p = 0.0006 (h). ns, not significant; *p = 0.0284; **p = 0.0012 (i). Data are presented as the mean ± SEM and analyzed using a two-sided unpaired Student t test (b, c) and two-way ANOVA followed by the Tukey’s post hoc test (e, f, g, h, i). The data represent three independent experiments with similar results. Source data are provided as a Source Data file.

Back to article page