Fig. 4: LNVS promotes the recruitment of antigen-specific T cells to the tumor in the presence of ultrasound. | Nature Communications

Fig. 4: LNVS promotes the recruitment of antigen-specific T cells to the tumor in the presence of ultrasound.

From: Tuning the fluidity and protein corona of ultrasound-responsive liposomal nanovaccines to program T cell immunity in mice

Fig. 4: LNVS promotes the recruitment of antigen-specific T cells to the tumor in the presence of ultrasound.

a The biodistribution of liposomal nano-sonosensitizer (LNS) and LNV containing sonosensitizer (LNVS) in the tumor at indicated time points (n = 5 mice per group). b C57BL/6 mice were inoculated subcutaneously with 1 × 106 MC38 tumor cells on day 0 and vaccinated with the indicated formulations on days 10 and 15. Ultrasound was applied to the tumor region on days 11 and 16. c Shown are the levels of chemokines in the tumor on day 18 (n = 4 mice per group). d Representative scatter plots for intratumoral DC activation and (e, f) expression levels of CD80 and CD86 on DC (n = 4 mice for WT and n = 5 mice for all other groups). g, h The frequency of antigen-specific CD8α+ T cells in the tumor on day 18 and representative scatter plots (n = 5 mice for DOPE and n = 6 mice for all other groups). Data were analyzed by one-way ANOVA (e, f) or two-way ANOVA (g) with Tukey’s multiple comparisons post-test. Data represent mean ± SEM. Figure 4b, created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

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