Fig. 7: Computer simulations of the proposed model of evolved HR.
Parameters used for these simulations are e = 5 × 10−7 μg/cell; vmaxN = 1.0, vmaxS = 0.5, vmaxH = 1.0 per cell per hour; vmin = −0.01 per cell per hour; K = 1; k = 1; MICN = 1.0, MICS = 8.0, MICH = 2.0 µg/ml; da = 0 µg/hour; µns = 1×10-8, µsn = 1 × 10-8, µsh = 1 × 10−3, µhs = 1 × 10−3 per cell per hour. The variables N, S, and H are, respectively, the wildtype E. coli MG1655, SCVs, and the heteroresistant bacteria in cells/mL. Note, points which do not appear to have error bars have standard deviations which are too small to be graphed. One hundred independent runs of the models were carried out in each condition. A H and S selection from an initial sensitive population. Parameters are N = 1 × 106 cells/mL; A = 3 μg/mL. B H selection from an initial SCV population in the absence of the drug. Parameters are S = 1 × 106 cells/mL; A = 0 μg/mL. C S selection from an initial H population in the presence of the drug. H = 1 × 106 cells/mL; A = 3 μg/mL. D Changes in the average MIC of the system over the simulations shown (A, B, and C).
