Fig. 4: A subpopulation of muscle progenitors co-expressing Pax3 and Hand2 aggregates at the proximal region of emu forelimb buds. | Nature Communications

Fig. 4: A subpopulation of muscle progenitors co-expressing Pax3 and Hand2 aggregates at the proximal region of emu forelimb buds.

From: Immobilization secondary to cell death of muscle precursors with a dual transcriptional signature contributes to the emu wing skeletal pattern

Fig. 4

a–e HCR for Pax3 and Hand2 and immunostaining for MF20 in emu forelimb buds at stages 19 (a, n = 2), 23 (b, n = 3) and 25 (c–e, n = 3). c’ Enlarged images indicated in (c). Arrowheads indicate fragmented nuclei. Note that transcripts of both Pax3 and Hand2 were observed in aggregated cells (arrows). d, Note that MF20 signals (arrows) were not detected in the aggregated cell population (a dotted circle). e HCR for Pax3 and Hand2 in the aggregated cell population at the proximal region of stage 25 emu forelimb buds (arrows). Note that transcripts of Pax3 and Hand2 are co-localized. Arrowheads indicate fragmented nuclei. f–h HCR for Pax3 and Hand2 in chicken forelimb buds at stages 19 (f, n = 3), 23 (g, n = 4) and 25 (h, n = 2). h’ Enlarged images indicated in (h). Unlike emu forelimb buds, no aggregated muscle progenitors are observed at the proximal part of chicken forelimb buds at stages 23 or 25. Scale bars, 100 μm (a–d, c’, h’), 20 μm (e), 200 μm (f–h). Panels of (a), (c, c’) and (g) are flipped horizontally.

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