Fig. 1: MPAL is comprised of a common transcriptomic signature and heterogenous transcription-immunophenotypic associations.
From: Multiomic single cell sequencing identifies stemlike nature of mixed phenotype acute leukemia
a Schematic depicting sample workflow. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en). b RNA-derived UMAP from comprehensive SC CITE-seq analysis of 71,579 cells from 12 patients. Cells are color-coded by cell lineage/type as determined by gene expression data (left) and by individual patient (right). Source Data are provided as a Source Data file. c Heatmap of scaled expression values for top 10 most upregulated genes for each transcriptionally defined cell type as identified in (b). Source Data are provided as a Source Data file. d RNA-derived UMAP from (b). Cells are annotated based on transcriptionally defined cell populations, clustered by the expression of cell-surface immunophenotypic protein expression into 13 immunophenotype-defined clusters, and then color-coded based on cluster. Source Data are provided as a Source Data file. e Heatmap of scaled expression values for top 10 most upregulated genes in each of the 13 immunophenotypic subpopulations from (d). Source Data are provided as a Source Data file. f RNA-derived UMAP from 2594 cells from Patient 11. Cells are color-coded based on expression of CD34 (left) and CD33 (right). Source Data are provided as a Source Data file. g Heatmap of scaled expression values for top 10 most upregulated genes for the CD34-positive cell population (left columns) and the CD33-positive cell population (right columns) from Patient 11. Source Data are provided as a Source Data file. h RNA-derived UMAP from 6100 cells from Patient 2. Cells are color-coded based on expression of CD34 (left) and CD33 (right). Source Data are provided as a Source Data file. i Heatmap of scaled expression values for top 10 most upregulated genes for the CD34-positive cell population (left columns) and the CD33-positive cell population (right columns) from Patient 2. Source Data are provided as a Source Data file.
