Fig. 5: Correlation of RNA isoform choice with chromatin accessibility and TFs during RPC development. | Nature Communications

Fig. 5: Correlation of RNA isoform choice with chromatin accessibility and TFs during RPC development.

From: Simultaneous profiling of RNA isoforms and chromatin accessibility of single cells of human retinal organoids

Fig. 5

a Heatmaps of the major isoform choices in RPCs and cones. b Pie chart showing the ratio of isoform alternative splicing events (SE) correlated with differentially accessible region (DAR), n = 2292. c Venn diagram showing the overlaps between predicted TFs binding to alternative splicing (AS) region and known AS-related TFs. d Venn diagram showing the overlaps between predicted TFs binding to alternative splicing (AS) region and fate determinators. e Co-localization analysis of TFs related to splicing and fate determination at splicing sites in the diagrams (c, d) (n = 21). f-g Schematic representation of MTHFD2 (f) and MAP3K3 (g) locus undergoing SE with concurrent changes in chromatin accessibility. Significance was computed using two-sided t-test. Data are presented as mean values +/- SEM (n = 3 duplicate samples). h Integration of transcription factor expression changes and motif analysis at variable splicing sites in MTHFD2 and MAP3K3. Source data are provided as a Source Data file.

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