Fig. 6: CENEXIN1 but not the shorter ODF2(iso6) isoform regulates the PML-NBs translocation of FBF1.

a Western blot showing changes of CENEXIN1, FBF1, and senescence markers in IR-treated RCTE cells. b Immunofluorescence images of RCTE cells expressing NG tagged-CENEXIN1 or short isoform ODF2 (iso6) with or without IR treatment. FBF1 (red) was immunostained by antibody. CENEXIN1 or ODF2 (iso6) was shown by NG direct fluorescence (green). Scale bar, 10 μm. c Nuclear PML-NBs translocation of CENEXIN1 and biotinylated proteins in control or APEX2-tagged CENEXIN1 over-expression RCTE cells with or without IR treatment. Endogenous CENEXIN1 (green) and PML (cyan) were labeled with antibody, respectively. Biotinylated proteins were labeled with streptavidin (red). Scale bar, 10 μm. Immunofluorescence images showing nuclear translocation of FBF1 (d) and quantitation of PML-NBs number (e) in IR-treated control or shCENEXIN1 RCTE cells (n = 40 cells). γ-tubulin (white) labels ciliary base. Scale bar, 10 μm. Immunofluorescence images showing the rescuing effect of re-expressing NG-tagged CENEXIN1 or ODF2 (iso6) (f) and quantitation of PML-NBs number (g) in IR-treated control or CENEXIN1^−/− RCTE cells (n = 40 cells). CENEXIN1 or ODF2 (iso6) was shown by NG direct fluorescence. Endogenous CENEXIN1 (green), FBF1 (red) and PML (cyan) were labeled with antibody, respectively. Scale bar, 10 μm. All results from n = 3 independent experiments. Data are the mean ± SEM. Statistical significance was determined using one-way ANOVA. Three experiments were repeated independently with similar results (a–c). Source data are provided as a Source Data file.