Fig. 3: Confirmation of Aβ_1-42 presence on Aβ-binding monocytes in blood.

PBMCs from CU individuals were FACS sorted based on CCR2 and CD14 expression. a Western blots (n = 2) showed substantial Aβ_1-42 on CCR2⁺⁺CD14⁺ monocytes, smaller amounts on CCR2⁺CD14⁺ monocytes, and no signal on CCR2^-CD14^- lymphocytes. b SELDI-TOF mass spectrometry (n = 2) confirmed diverse Aβ_1-42 species, including dimers, in CCR2⁺⁺CD14⁺ and CCR2⁺CD14⁺ monocytes, but not in CCR2⁻CD14⁻ lymphocytes. c TQ-MS (n = 3) identified Aβ-specific fragments (28–42) on various cell types, including CCR2⁺⁺CD14⁺ and CCR2⁺CD14⁺ monocytes, and CCR2⁻CD14⁻ lymphocytes, with fragment (16–27) also detected abundantly.