Fig. 6: Transcriptional machinery consolidates recruitment of ZIC2 over primed-specific enhancers.
From: ZIC2 and ZIC3 promote SWI/SNF recruitment to safeguard progression towards human primed pluripotency
a Enrichment of total RNAPII, Ser5p-RNAPII and BRD4 over ZIC2-bound regions. RPGC reads per 1x genome coverage. Enrichment of Ser5p-RNAPII to total RNAPII is also shown. b Schematic of experiments designed to test the effect of inhibiting various components of transcription and its machinery on recruitment of ZIC2. c Effect of treatment with triptolide (Trp) for 4 h on ZIC2 occupancy in primed hESCs. d Effect of treatment with flavopiridol (FLV) and STM2457 for 4 and 24 h, respectively, on ZIC2 occupancy in primed hESCs. c, d CPB = normalized counts per bin. e Comparison of 4 h FLV and 24 h STM2457 treatment to 4 h Trp treatment on ZIC2 occupancy over C3 loci. Results were computed from two independent biological replicates. f Comparison of Trp treatment for 1 and 4 h on ZIC2 occupancy over C3 loci. ChIP-seq data for the treatment of primed hESCs with triptolide for 4 h was computed from two independent biological replicates, and data for treatment with triptolide for 1 h was computed from one replicate. e, f Median values are shown, and boxplots represent the inter-quartile range. Whiskers are calculated using the Tukey method. g Genome browser tracks showing differential effects of ZIC2 occupancy over primed-specific enhancers and bivalent regions upon treatment with triptolide in primed hESCs. Source data are provided as a Source Data file.
