Fig. 4: Structural characterization of SthK with mutated PIP2 binding sites.
From: PIP2 inhibits pore opening of the cyclic nucleotide-gated channel SthK
a Density map of SthK R120A in 3:1 DOPC:POPG + 10 % PIP2 nanodiscs. Protein density is shown in light blue, lipid densities in yellow and PIP2 density in blue. Zoom focuses on the lipid densities. b Density map of SthK R120A/R124A in 3:1 DOPC:POPG + 10 % PIP2 nanodiscs. Protein density is shown in light green and lipid densities in yellow. Zoom focuses on the lipid densities. c Comparison of lipid densities in cryo-EM maps of WT SthK (top), SthK R120A (middle), and SthK R120A/R124A (bottom). Density of PIP2 is shown in blue and of the additional lipid bound along the S6 helix in yellow.
