Fig. 2: Morphology and dsx sex-specific transcript expression. | Nature Communications

Fig. 2: Morphology and dsx sex-specific transcript expression.

From: Population suppression by release of insects carrying a dominant sterile homing gene drive targeting doublesex in Drosophila

Fig. 2: Morphology and dsx sex-specific transcript expression.The alternative text for this image may have been generated using AI.

a Schematic representation of the male- and female-specific dsx transcripts. Red arrows are primers designed for male- or female-specific transcript diagnostic PCR. The PCR product for the female dsx transcript is 713 bp, and the product for the male transcript is 352 bp. b Pictures of wild-type (+/+) flies, female drive carriers (D/+), and nonfunctional (r2) resistance allele females are shown. dominant r2/+ refers to dominant sterile r2 individuals, recessive r2/+ refers to recessive sterile r2 individuals. D/+, dominant r2/+, and recessive r2/+ females were homozygous for the nanos-Cas9-nanos allele. c Diagnostic PCR on cDNA using female-specific primers (top) and male-specific primers (bottom).

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