Fig. 1: Characterization of mutants vig1a and vig1b, and isolation of VIG1.
From: A point mutation in VIG1 boosts development and chilling tolerance in rice
a Seedling morphology of WT and vig1a. Scale bars, 1 cm. b, c Investigation of the shoot length (b) (n = 20 independent seedlings), and seminal root length (c) (n = 20 independent seedlings) after one to seven days growth after germination (DAG). d–f Phenotypic comparison of the chilling tolerance (d), whole plant (e) and panicle length (f) between WT and vig1a. Scale bars, 4 cm (d), 15 cm (e) and 2 cm (f). The chilling tolerance for 14-day-old WT and vig1a was repeated three times with 80–96 seedlings per biological replicate and obtained similar results. The heading date for WT and vig1a plants was investigated three plots with 40–50 plants per plot. g–j Investigation of the survival rate (g) (n = 3 biologically independent experiments), heading date (h) (n = 3 biologically independent experiments), plant height (i) (n = 30 independent plants), and grain number per panicle (j) (n = 30 independent panicles). k Map-based cloning of gene VIG1. The black boxes indicate genes within the region between markers M5 and M6. The red box represents the candidate gene OsbZIP01 (LOC_Os01g07880). The red letters indicate a guanine to adenine (G-A) point mutation occurred at the second exon of OsbZIP01 in vig1a, which results in the 121st arginine to histidine (R-H) substitution of its encoding protein. l Seedling growth phenotype of WT and vig1b. Scale bars, 1 cm. m, n Investigation of the shoot length (m) (n = 20 independent seedlings), and seminal root length (n) (n = 20 independent seedlings) after one to seven days growth after germination (DAG). Values are the mean ± SD (two-tailed t-test, ***P < 0.001). Source data are provided as a Source Data file.
