Fig. 4: Entinostat treatment altered abundance of myeloid populations in PBMC.

a Heatmap of the normalized expression for all markers used during FlowSOM from baseline and post-treatment samples using a Myeloid cell-oriented oriented CyTOF panel. N = 28 paired patient sample between baseline and C1D1, and n = 8 paired samples between C1D1 and C2D1. Cell subtypes listed on the right were assigned based on differential expression of the markers listed across the bottom. B cells, cDC1- classical dendritic cell type 1, cDC2-classic dendritic cell type 2, DNT- double negative T cells (neither CD4 nor CD8 expressing T cells), early myelocytes, Granulocytes, Non-classic monocytes, pDC-plasmacytoid dendritic cell, pre-DC - preDendritic cells (preDC)/Classic monocytes, Tc - Cytotoxic T cells, Th- Helper Tcells. b Bar plot of the proportion of myeloid cell populations at baseline, after Entinostat lead-in (C1D1), and post combination (C2D1). c Proportions of preDC/Classic monocytes (p = 0.007); early myelocytes (p = 0.001), pDC (p < 0.001), and cDC2 (p < 0.001) at different time points. Each line corresponds to one patient. Red line corresponds to a non responder; blue line corresponds to a responder. d Evaluation of CLEC9A (p = 0.016), CD103 (p < 0.001), and Arginase (p < 0.001) expression across cellular groups in the myeloid partition at given time points. e–g, Evaluation of CD40, CCR5, and CCR2 expression across cellular groups in the myeloid partition at given time points P-values from paired two-sided Wilcoxon test between time points and the mean of the difference between time points are shown. Statistically significant P value is shown as follows: *P < 0.05. For all panels: N = 28 paired patient sample between baseline and C1D1, and n = 8 paired samples between C1D1 and C2D1. Box plots show the median and upper and lower quartiles and whiskers extend to 1.5× the interquartile range. Box plots show the median and upper and lower quartiles and whiskers extend to 1.5× the interquartile range. Source data are provided as a Source Data file. IL-6, interleukin 6; IP10, interferon γ-induced protein 10 kDa; MCP-1, Monocyte Chemoattractant Protein-1; MDC, Macrophage-derived chemokine; MIP-1b, Macrophage inflammatory protein-1 beta; sCD40L, soluble CD40 Ligand; NR, non responders; R, responders.