Fig. 3: CPR-6 activity is modified with aging by a temperature-sensitive modulator.

a An analysis of the age-dependent changes in CPR-6 activity patterns from embryonic development through larval development and adulthood (n = 3). b Mixing homogenates of L4 larvae (lane 3) and day 5 old worms (lane 4) shows changes in the intensities of the different bands. Bands 2 and 3 are largely intensified whereas bands 4 and 5 are greatly weakened (lane 5). These effects are abolished when the L4 homogenate was heat inactivated (lane 6) or when the homogenates were not incubated prior to the analysis (lane 7) (n = 3). c Different mixing ratios indicate that 25% of L4 homogenate is sufficient to greatly reduce the intensities of bands 4 and 5 (lane 7) (n = 2). d Protein identification by mass spectrometry indicates that CPR-6 is the sole protein that appears in all five bands. e–h Analysis of proteins that sediment with each CPR-6-containg band shows an enrichment of cytosolic and mitochondrial proteins in all bands. Interestingly, lysosomal proteins were less abundant.