Fig. 5: cpr-6 RNAi modulates the proteome of larvae and adult worms.

a, b Mass spectrometry indicates that the knockdown of cpr-6 by the 3’UTR RNAi leads to an increase in the levels of 77 proteins and to decreased levels of 52 proteins in L4 larvae. 204 proteins showed increased levels and 42 exhibited reduced amounts in day 5 adult worms upon the knockdown of cpr-6. c, d Clustering of the identified proteins by biological function shows an enrichment of proteins that are involved in splicing, nuclear import and organization in day 5 old worms. e The most prominent proteins that showed at least two-fold elevated (upper table) or decreased (lower table) levels in day 5 old CL2006 worms (p-value was calculated using T-test, 2 tails, unpaired). f A paralysis assay indicates that smk-1 is crucial for the cpr-6 RNAi-mediated protection from Aβ proteotoxicity. g Three independent repeats of the paralysis assay as in (f). Bars represent average daily rates of paralysis in the population ± SEM (n = 3). Statistical test used: unpaired, one-tailed Student’s t-test (*p < 0.05, **p < 0.01).