Fig. 2: Dopamine 1 receptor localizes to adenylyl cyclase isoforms positive compartments.
From: Spatial organization of adenylyl cyclase and its impact on dopamine signaling in neurons
a, b Maximum intensity Z-projection of confocal microscopy images of MSNs expressing FLAG-D1R and HA-AC3 (a) or HA-AC5 (b) from n = 4 independent experiments. Scale bar = 10 μm. c Representative live cell spinning disk confocal images of MSNs transfected with AC9-GFP and FLAG-D1R and treated with 10 μM dopamine (DA) at 0 min. Surface FLAG-D1R was labeled with Alexa Fluor 555-coupled anti-FLAG antibody for 15 min before imaging. Scale bar = 5 μm. d Quantification of surface labeled FLAG-D1R accumulation at segmented AC9-GFP-positive endosomes after vehicle (Veh) or 10 μM DA addition. Data are shown as mean ± s.e.m. from n = 5 independent experiments; (33–35 cells total/condition). Source data are provided as a Source Data file.
