Fig. 5: Phenotypic and genomic characterisation of phage-treated Paer09 isolates.

a Efficiency of plating (EOP) of five bacteriophages (phages) (FJK, FJK.R9-15, FJK.R9-30, MK, and MK.R3-15) for 48 Paer09 strains treated with individual phages (FJK, FJK.R9-15, FJK.R9-30). The EOP is defined as the concentration ratio of a phage on the phage-treated isolate (numerator) and the naive Paer09 strain (denominator; Control). An EOP above 10 was considered as increased efficiency (green), while an EOP of 0.1 to 10 was ranked as unchanged efficiency (grey). A reduced efficiency was defined as an EOP between 0.001 and 0.1 (light blue). When no individual plaques were visible or the EOP was equal to or under 0.001 the isolate was determined resistant to the phage (blue). Mutation-associated protein of each strain with amino acid change in parentheses. A lack of labelling refers to isolates in which no apparent mutation could be identified. b Illustration of the cumulative number of isolates by mutant protein. c Illustration of the number of isolates by mutant protein according to the treatment group (phage FJK; phage FJK.R9-15; phage FJK.R9-30). Optical density (OD600) measurements of a three-day co-incubation of planktonic naive Paer09 strain with phage FJK (d), phage FJK.R9-15 (e) and phage FJK.R9-30 (f) at an MOI of 0.001. The thin black lines represent each individual replicate (n = 8), and the thick coloured line represents the average. For phages FJK.R9-15/30 the completely suppressed replicate was excluded from the average calculation. g Illustration of the averaged co-incubation curves for phages FJK (grey line; panel d), FJK.R9-15 (violet line; e) and FJK.R9-30 (yellow line; f) over three days in comparison to a growth control (dotted black line) and negative control (black line). Source data are provided as a source data file.