Fig. 2: Scope of fumarate and maleate substrates. | Nature Communications

Fig. 2: Scope of fumarate and maleate substrates.

From: Cooperative chemoenzymatic and biocatalytic cascades to access chiral sulfur compounds bearing C(sp3)–S stereocentres

Fig. 2: Scope of fumarate and maleate substrates.

Reaction conditions: (Z)-1 and/or (E)-1 (0.1 mmol), ENE-101 (25 mg, 65.8 U/mg, 1.2 mol‰), GDH-101 (12.5 mg, 32.0 U/mg), D-glucose (0.5 mmol), NADP+ (5 μmol), 1.9 mL KPBS (250 mM, pH 7.0), DMSO (100 μL), 30 oC, 24 h. c = conversion; y = isolated yield; ee = enantiomeric excess. Isolated yields were not determined when the conversions were less than 50%. Turnover number (TON) was calculated as the moles of substrate that were converted by per mole of ENE-101 biocatalyst.

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