Fig. 6: Scope of bio-chemo/bio-bio cascades for β-hydroxysulfide synthesis. | Nature Communications

Fig. 6: Scope of bio-chemo/bio-bio cascades for β-hydroxysulfide synthesis.

From: Cooperative chemoenzymatic and biocatalytic cascades to access chiral sulfur compounds bearing C(sp3)–S stereocentres

Fig. 6: Scope of bio-chemo/bio-bio cascades for β-hydroxysulfide synthesis.

a Scope of the two-step one-pot bio-chemo/bio-bio cascade. Reaction conditions: 5 (0.1 mmol), ENE-101 (25 mg, 65.8 U/mg, 1.2 mol‰), GDH-101 (12.5 mg, 32.0 U/mg), D-glucose (0.5 mmol), NADP+ (5 μmol), 1.9 mL KPBS (250 mM, pH 7.0), DMSO (100 μL), 30 oC, 1.5, 2 or 3 h; then MeOH (1 mL), NaBH4 (0.5 mmol), room temperature, 1 h; or ADH-153 (50 mg, 3.3 U/mg, 23.8 mol‰), 30 oC, 18 h; or ADH-19 (50 mg, 2.8 U/mg, 3.8 mol‰), 30 oC, 18 h. b Scope of the one-step one-pot bio-bio cascade. Reaction conditions: 5 (0.1 mmol), ENE-101 (25 mg, 65.8 U/mg, 1.2 mol‰), ADH-153 (50 mg, 3.3 U/mg, 3.8 mol‰) or ADH-19 (50 mg, 2.8 U/mg, 3.8 mol‰), GDH-101 (12.5 mg, 32.0 U/mg), D-glucose (0.5 mmol), NADP+ (5 μmol), 1.9 mL KPBS (250 mM, pH 7.0), DMSO (100 μL), 30 oC, 18 h. c = conversion; y = overall isolated yield; dr = diastereomeric ratio; ee = enantiomeric excess. Isolated yields were not determined when the conversions were less than 50%.

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