Table 1 Optimization of reaction conditions^a

^aReaction conditions: (Z)-1aa and/or (E)-1aa (0.02 mmol), ENE (5.0 mg, 1.2 mol‰), GDH (2.5 mg), D-glucose (0.1 mmol), NADP⁺ (1 μmol), 950 μL KPBS (250 mM, pH 7.0), DMSO (50 μL), 30 ^oC, 24 h. ^bDetermined by chiral HPLC analysis. ^cNAD⁺ was used instead of NADP⁺ as the cofactor. ^dThe reaction was performed at 37 ^oC. ^eIsolated yield of 2aa when 0.1 mmol of 1aa was used. ^fThe ee value of 2aa when 0.1 mmol of 1aa was used. ENE-101 (65.8 U/mg), ENE-102 (5.4 U/mg), ENE-103 (3.0 U/mg), ENE-105 (9.0 U/mg), ENE-107 (101.0 U/mg), ENE-108 (6.0 U/mg), ENE-109 (6.1 U/mg), GDH-101 (32.0 U/mg), GDH-5 (51.2 U/mg), GDH-8 (1.6 U/mg).

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