Fig. 1: Evidence for DNG function in pollen and summary of experimental approach.
From: Potent pollen gene regulation by DNA glycosylases in maize
a Expression of genes with teM in anther and tassel. The X-axis indicates the number of genes in each of the ten tissues of ref. 34 which have teM and TPM values of at least 100. This analysis only includes high-confidence genes defined as genes that do not overlap with TE annotations and are part of the “core gene” set35. b Top panel, pollen from an mdr1 /Mdr1 dng102/Dng102 double heterozygous plant, segregating four haploid genotypes. The bottom panels show large and small pollen grains from a representative plant. Pollen from all six double heterozygous plants imaged exhibited a small pollen (sp) phenotype (Supplementary Fig. 2). Size bar = 100 μm. c Schematic of single-pollen mRNA-seq method. Individual libraries were prepared and sequenced for each pollen grain. Capital indicates WT allele, lowercase mutant, red double mutant. d Unsupervised clustering of single-pollen transcriptomes based on Pearson correlation across the entire dataset (all by all). Warmer colors indicate stronger correlations between transcriptomes. The top two rows indicate mdr1 and dng102 genotypes inferred from SNPs linked to the two loci derived from RNA-seq data, which were scored independently of the transcriptome correlation analysis. Genotypes: black is mutant, light gray is wild-type, and dark gray is ambiguous.
