Fig. 1: LD accumulation coincides with ciliary loss in VECs during atherosclerosis progression. | Nature Communications

Fig. 1: LD accumulation coincides with ciliary loss in VECs during atherosclerosis progression.

From: Lipid droplets sequester palmitic acid to disrupt endothelial ciliation and exacerbate atherosclerosis in male mice

Fig. 1

a, b Representative ORO staining and quantitative analysis of the whole aorta from 8-week-old ApoEKO mice fed a normal chow (NC) or a high-fat diet (HFD) for 0, 8, and 16 weeks (n = 4 mice). Scale bar, 5 mm. c En face immunofluorescence images of the inner curvature of the aortic arch VECs stained with antibodies against VE-cadherin (white) and ac-tubulin (magenta). LDs were stained with BODIPY (green), and nuclei were stained with DAPI (blue). Scale bar, 10 μm. d, e Quantification of BODIPY staining (d) and ciliation (e) of VECs of the aortic arch shown in (c) (n = 10 mice). f, g Scatter plots showing the relationship between ciliary signal and LD signal of randomly chosen ciliated VECs of the inner curvature of the aortic arch (n = 10 mice for each group). Male mice were used for this study. Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with post hoc analysis. Source data are provided as a Source Data file.

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