Fig. 1: Peroxidase activity of KlebCP on a small molecule substrate.

The figure shows the formation of resorufin resulting from H₂O₂-dependent oxidation of 100 µM Amplex Red by 0.1 μM KlebCP. The reactions were conducted at 30 °C in 50 mM Tris-HCl buffer, pH 8.0, containing 300 mM NaCl. Note that H₂O₂ was introduced to the reaction mixtures 3 times at 0, 2 and 4 min of the experiment (each time to 100 µM final concentration). Also note that the 100 µM Amplex Red was not consumed after two additions of 100 µM H₂O₂, whereas the added H₂O₂ is fully depleted; this shows that a considerable fraction of the added H₂O₂ is removed by the catalase activity of KlebCP. Progress curves represent the average result of three independent experiments (n = 3), whereas error envelopes indicate standard deviation between replicates. Source data are provided as a Source Data file.