Fig. 6: Reverse correlation between YAP activation and HBV replication in hepatocytes of CHB patients.

a snRNA-seq analysis of the hepatocyte population. t-SNE plots showed relative distribution of HBV transcript levels, scGSVA scores of YAP conserved signature, and HNF4A transcript levels in hepatocyte subsets. b Immunostaing was performed in liver biopsies obtained from two IA patients. The right panel depicted the proportion of nuclear-localized YAP in each cell under the specified field of view, categorized by HBsAg positivity and negativity. (n represented the count of individual cells within field of view). Data were represented as box plots(median with interquartile, upper-limit, and lower-limit). c–e Comparison of the transcript levels of indicated genes (YAP, HNF4A, and TGFβ1) in GSE65359 between IA and IT patients. The data are represented as mean ± SD. P value by two-tailed Student’s t-test (b–e). Source data are provided as a Source Data file. (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).