Fig. 2: Cellular changes associated with hyperactivity of MPOA glutamatergic neurons. | Nature Communications

Fig. 2: Cellular changes associated with hyperactivity of MPOA glutamatergic neurons.

From: Excitation-inhibition imbalance in medial preoptic area circuits underlies chronic stress-induced depression-like states

Fig. 2

a Schematic whole-cell recording from MPOA Vglut2+ neurons in slice preparations. b Left, example recorded traces of spontaneous spikes from a control (gray) and CRS (red) neuron. Right, mean ISIs in control and CRS groups (n = 10 and 10 cells, respectively, from three animals in each group). ***p = 0.0044, two-tailed Mann–Whitney test. c Left, trace of bursting firing in an example Vglut2+ neuron. Right, percentage of bursting firing cells. d Membrane potential responses to step-current injections in a control (gray) and CRS (red) neuron. Scale: 50 mV, 200 ms. e Average input–output function of Vglut2+ neurons in control and CRS groups (n = 9 and 10 cells, respectively, from three animals in each group). **p < 0.01, ****p < 0.0001, two-tailed repeated measures two-way ANOVA with Sidak’s test. f Recorded traces of sEPSCs in example Vglut2+ neurons in control (gray) and CRS (red) slices. Scale: 20 pA, 200 ms. g, h Mean inter-event intervals (g, IEIs) and mean amplitudes (h) of sEPSCs in control and CRS slices (n = 9 and 10 cells, respectively, from three animals in each group). ****p < 0.0001 (g) and **p = 0.007 (h), two-tailed Mann–Whitney test. i–k Similar to (f–h) but for sIPSCs (n = 9 and 10 cells for control and CRS, respectively, from three animals in each group). Scale: 20 mV, 200 ms. N.S., p = 0.3154 (j) and p = 0.8421 (k), two-tailed Mann–Whitney test. l Whole-cell recording from MPOA Vgat+ neurons. m Mean ISIs of Vgat+ neurons in control and CRS groups (n = 7 and 7 cells, respectively, from two animals in each group). N.S., p = 0.535, two-tailed Mann–Whitney test. n Average input–output function of Vgat+ neurons in control and CRS groups (n = 7 and 7 cells, respectively, from three animals in each group). p = 0.5337, two-tailed repeated measures two-way ANOVA. o, p Mean IEIs (o) and amplitudes (p) of sEPSCs in Vgat+ neurons in control and CRS slices (n = 7 and 7 cells, respectively, from three animals in each group). N.S., p = 0.9802 (o) and p = 0.9767 (p), two-tailed Mann–Whitney test. q, r Similar to (o, p) but for sIPSCs (n  = 7 and 7 cells for control and CRS, respectively, from three animals in each group). N.S., p = 0.62 (q) and p = 0.71 (r), two-tailed Mann–Whitney test. Error bar represents s.d. in (b), (g, h), (j, k), (o–r) and s.e.m. in (e), (n). For exact p values, see Source Data.

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