Fig. 3: Silencing MPOA glutamatergic neurons alleviates depressive-like behaviors. | Nature Communications

Fig. 3: Silencing MPOA glutamatergic neurons alleviates depressive-like behaviors.

From: Excitation-inhibition imbalance in medial preoptic area circuits underlies chronic stress-induced depression-like states

Fig. 3

a Schematic of viral injection and chemogenetic inhibition of MPOA Vglut2+ neurons in the expression phase. b Percentage sucrose water consumption in SPT in mCherry-expressing or hM4Di-expressing CRS animals. c Immobility time in TST. d Immobility time in FST. e Percentage time spent in open arms in EPM test. f Percentage time spent in the center in OFT. g Social preference index in the sociability test. Statistics, ***p = 0.0006 (b), ***p = 0.0005 (c), **p = 0.0027 (d), ****p < 0.0001 (e), ****p < 0.0001 (f), ****p < 0.0001 (g), two-tailed Mann–Whitney test, n = 12 animals for each group. h–n Similar to (a–g) but for chemogenetic silencing of MPOA Vglut2+ neurons during the CRS induction phase. Statistics, ***p = 0.0001 (i), ***p = 0.0007 (j), ***p = 0.0001 (k), ****p < 0.0001 (l), ***p = 0.0002 (m), ***p = 0.0001 (n), two-tailed Mann–Whitney test, n = 12 animals for each group. All error bars represent s.d. For exact p values, see Source Data. (a) and (h) were created with BioRender.com.

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