Fig. 6: Reduced MPOA GABAergic activity following chronic social defeat stress. | Nature Communications

Fig. 6: Reduced MPOA GABAergic activity following chronic social defeat stress.

From: Excitation-inhibition imbalance in medial preoptic area circuits underlies chronic stress-induced depression-like states

Fig. 6

a Schematic chronic social defeat stress. b Percentage sucrose water consumption. c Immobility time in TST. d Immobility time in FST. e Percentage time spent in opened arms of EPM. f Percentage time spent in the center in OFT. g Social preference index. Statistics, ****p < 0.0001 (b), ***p = 0.0002 (c), ****p < 0.0001 (d), ***p = 0.0001 (e), ***p = 0.0002 (f), ***p = 0.0002 (g), two-tailed Mann–Whitney test, n = 9 mice in each group. Data are presented as mean ± s.d. h Schematic optrode recording in MPOA of SDS animals. i, j Raster plots of spontaneous spikes of eight ChR2-tagged Vgat+ neurons in control (i) and CRS (j) animals. Insets are superimposed spike waveforms for each unit. k, l Mean spontaneous spike rates of all the recorded Vgat+ (k) and Vglut2+ (l) neurons in control and CRS groups (k, n = 87 and 75 cells, respectively, from three animals in each group, ****p < 0.0001, two-tailed unpaired t-test; l, n = 80 and 48 cells, respectively, from three animals in each group, p = 0.4268, unpaired t-test). m Whole-cell recording from Vgat+ or Vglut2+ neurons after SDS treatment. n Example membrane potential responses to step-current injections in a control (gray) and SDS (red) Vglut2+ neuron. o Average input–output function of Vglut2+ neurons in control (gray) and SDS group (n = 7 and 7 cells, respectively, from three animals in each group). p, q Excitability of Vgat+ neurons in the control (gray) and SDS (red) group (n = 9 and 8 cells, respectively, from three animals in each group). r Schematic chemogenetic activation of Vgat+ neurons. s Percentage sucrose water consumption in mCherry- and hM4Di-expressing animals. t Immobility time in TST. u Immobility time in FST. v Percentage time spent in open arms of EPM. w Percentage time spent in the center in OFT. x Social preference index. Statistics, **p = 0.0079 (s), ***p = 0.0079 (t), *p = 0.0159 (u), **p = 0.0079 (v), *p = 0.0476 (w), *p = 0.0317 (x), two-tailed unpaired Mann–Whitney test, n = 5 mice for each group. Error bars represent s.e.m. in (o), (q) and s.d. in the rest of the panels. For exact p values, see Source Data. (a) and (h) were created with BioRender.com.

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