Fig. 7: The MPOA E-I balance regulates depressive-like behaviors. | Nature Communications

Fig. 7: The MPOA E-I balance regulates depressive-like behaviors.

From: Excitation-inhibition imbalance in medial preoptic area circuits underlies chronic stress-induced depression-like states

Fig. 7

a Schematic of viral injection and chemogenetic activation of MPOA GABAergic neurons in CRS animals. b Percentage sucrose water consumption in SPT with CNO injection in mCherry- and hM3Dq-expressing mice. d Immobility time in FST. e Percentage time spent in open arms of EPM. f Percentage time spent in the center in OFT. g Social preference index. Statistics, *p = 0.0159 (b), *p = 0.0238 (c), **p = 0.0079 (d), **p = 0.0079 (e), *p = 0.0238 (f), *p = 0.0238 (g), two-tailed Mann–Whitney test, n = 5 mice in each group. h Schematic of viral injection and chronic chemogenetic activation of MPOA glutamatergic neurons for 2 weeks. i–n Similar to (b–g) but for chronic activation of glutamatergic neurons. **p = 0.0012 (i), **p = 0.0012 (j), ***p = 0.0006 (k), ***p = 0.0006 (l), ***p = 0.0006 (m), **p = 0.0012 (n), two-tailed Mann–Whitney test, n = 7 mice in each group. o Schematic of viral injection and chronic chemogenetic suppression of MPOA GABAergic neurons. p–u Similar to (i–n) but for chronic suppression of GABAergic neurons. ***p = 0.0006 (p), ***p = 0.0006 (q), ***p = 0.0006 (r), ***p = 0.0006 (s), ***p = 0.0006 (t), **p = 0.0017 (u), two-tailed Mann–Whitney test, n = 7 mice in each group. All error bars represent s.d. For exact p values, see Source Data. (a) was created with BioRender.com.

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