Fig. 4: 10F-THF and DHP increase Ca²⁺ transients in HSN neurons.

a Traces of fluorescence (∆F/F) for 1000 s from transgenic animals with GCaMP5 expressed in the HSN neurons for the indicated genotypes and treatment with 40 nM 10F-THF or control buffer. Egg-laying events are denoted with red arrowheads. b The number of Ca²⁺ transients in HSN neurons for the indicated genotypes and concentrations of 10F-THF or control buffer. n values for control and 200 nM 10F-THF, respectively, are: wild type (13, 14); folr-1 (11, 11); gon-2 (12, 13). The n = 19 for control and 40 nM 10F-THF for all genotypes. c The number of transients in HSN neurons for the indicated genotypes and concentrations of DHP or control buffer. The n values for control and 200 nM DHP, respectively, are: wild type (13, 14); folr-1 (11, 10); gon-2 (7, 11). n = 19 for control and 40 nM DHP for all genotypes. n = Movies of individual animals to assess Ca²⁺ transients (b, c). Data are presented as mean ± SEM. Unpaired two-tailed Student’s t-tests were conducted for statistical analyses. Source data are provided as a Source Data file. ns not significant.