Fig. 6: Excellent preventive and curative effects of LNT/SPc complex on TMV. | Nature Communications

Fig. 6: Excellent preventive and curative effects of LNT/SPc complex on TMV.

From: Nanomaterial inactivates environmental virus and enhances plant immunity for controlling tobacco mosaic virus disease

Fig. 6

a Preventive effect of LNT/SPc complex in the laboratory. Tobacco seedlings were sprayed with LNT (100 mg/L) or LNT/SPc complex, and 50 µL TMV-GFP (20 µg/mL) was inoculated 48 h later. The ddH2O and SPc were also employed. Biomass was quantified (n = 4 biological replicates, 3 seedlings/replicate). The representative photos were acquired at 3 dpi, and the TMV-GFP relative expression was quantified via qRT-PCR at 2, 3 and 4 dpi. Significant differences indicated by t test (two-sided, 3 dpi: t = 3.06, df = 6, p = 0.022; 4 dpi: t = 3.44, df = 6, p = 0.014; *p < 0.05). b Curative effect of LNT/SPc complex in the laboratory. Seedlings were inoculated with TMV-GFP, then sprayed with LNT (100 mg/L) or LNT/SPc complex after 24 h. Biomass was quantified (n = 4 biological replicates, 3 seedlings/replicate). The representative photos were acquired at 3 dpi, and the TMV-GFP relative expression was quantified by qRT-PCR at 2, 3 and 4 dpi. Significant differences indicated by t test (two-sided, t = 3.20, df = 6, p = 0.019, *p < 0.05). c Control efficacy of LNT/SPc complex against TMV in the field. Tobacco seedlings were sprayed with LNT (40 mg/L) and LNT/SPc complex, respectively. The water and SPc were also applied. Each treatment contained three independent plots (Three replicates), and each plot was approximately 240 m2. The representative photos were taken at 14 d after the treatment. Significant differences indicated by t test (two-sided, 7 d: t = 2.85, df = 4, p = 0.047; 14 d: t = 3.73, df = 4, p = 0.020, *p < 0.05). d Agronomic traits of treated tobacco seedlings at 14 d. Each treatment included 35 randomly-selected seedlings. Different letters above each bar indicate significant differences at p < 0.05 as determined by one-way ANOVA with Tukey HSD test (Plant height: F3, 136 = 28.05, p < 0.001; Leaf width: F3, 136 = 14.35, p < 0.001; Leaf length: F3, 136 = 19.42, p < 0.001). a-d Bars represent the mean ± SEM. Source data are provided as a Source Data file.

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