Fig. 5: In vivo imaging of endothelial cell EV formation and cell fragmentation during AML. | Nature Communications

Fig. 5: In vivo imaging of endothelial cell EV formation and cell fragmentation during AML.

From: In situ visualization of endothelial cell-derived extracellular vesicle formation in steady state and malignant conditions

Fig. 5

a Representative calvarium Maximum Intensity Projection (MIP) of steady-state or AML-burdened Flk1-GFP mice. b MIP of time-lapse intravital microscopy analysis performed on individual blood vessels in steady state or AML-burdened mice (projection of merged images taken every 1 s for 5 min). c Quantification of EV formation by intravital microscopy of AML-burdened mice. Each data point represents the average number of EV formation events observed per 2 h of imaging per mouse (n = 8). d Quantification of Flk1-GFP+ EV diameter derived from intravital microscopy data. Each data point represents an individual EV (n = 37). e, f Example of Flk1-GFP+ cell blebbing and EV formation in AML-burdened mice. Timestamp presented as min:s. Error bars represent SEM. Statistical analysis: Unpaired Student’s two-tailed t-test, ns = p > 0.05, *p < 0.05, ***p < 0.001. At least three independent experiments were performed for all experiments. AML-burdened mice assessed at 18 days post-transplantation (DPT). For all microscopy images shown, green = Flk1-GFP, red = MLL-AF9 tdTomato and grey = SGH.

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