Fig. 3: Validation of a subset of interactions by orthogonal biochemical assays.

A Matrix displaying the overall recovery of synergies and antagonisms during validation. Interactions were categorized according to their Bliss score (>0: synergies; <0: antagonisms), irrespective of their absolute value. B Selected interactions (x-axis), including their screening score (right y-axis, black dots for each interaction), as well as their Bliss score obtained during validation (left y-axis, orange and blue bars, calculated from LDH-release). Bars indicate mean with SEM (based on at least three biological replicates, each with at least two technical replicates). Small insert (b) displays time-resolved fluorescence signal obtained in the screen (left panel, representative of the two biological replicates, error bars indicate standard deviation of three technical replicates) and quantification from LDH-release obtained in the validation (right panel, mean and SEM are indicated, blue bars depict a validated synergy, orange bars a validated antagonism). C As for panel B, but concerning bacterial growth as readout. Small insert (c) shows the time-resolved fluorescence signal for the displayed co-infection (proxy for bacterial growth, upper panel, representative replicate from the screening), as well as the validation of this co-infection pair by CFU quantification).